Journal: Nutrients

Article Title: Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV- neu T) in Mice with Transplanted Salivary Gland Carcinoma Cells

doi: 10.3390/nu12051417

Figure Lengend Snippet: Interferon (IFN)-γ and Interleukin (IL)-2 release from splenocytes treated with Neu peptides. The release of cytokines was assessed in splenocytes collected at sacrifice from treated mice after 96 h stimulation in vitro with Neu peptides. The results show the cytokine release induced by an irrelevant peptide (gag) subtracted from the IFN-γ or IL-2 release of each sample, in two experiments performed in duplicate (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001; one-way-ANOVA, Tukey’s multiple comparison). ( A , C ) show the release of IFN-γ or IL-2 when all peptides were used in the assay, while ( B , D ) show the contribution of each peptide to the T-cell cytokine release. Results represent three independent experiments of T-cell stimulation with Neu peptides.

Article Snippet: Cytokine release, IHC data, and distribution of cell survival were preliminarily verified using the Kolmogorov–Smirnov test and data sets were analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s test with GraphPad Prism 5.00.288.

Techniques: In Vitro, Comparison, Cell Stimulation

Journal: Nutrients

Article Title: Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV- neu T) in Mice with Transplanted Salivary Gland Carcinoma Cells

doi: 10.3390/nu12051417

Figure Lengend Snippet: Helper T cells infiltrating tumors in BALB- neu T mice after treatments. Tumor tissues from three mice in each group were analyzed with IHC for CD4 expression. ( A ) Positive cell count/field averaging 10 representative microscopic fields (mean ± SD, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; one-way-ANOVA, Tukey’s multiple comparison). ( B ) Representative digital images (20×), scale bar represents 100 µm. T H : helper T lymphocytes, CUR: curcumin.

Article Snippet: Cytokine release, IHC data, and distribution of cell survival were preliminarily verified using the Kolmogorov–Smirnov test and data sets were analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s test with GraphPad Prism 5.00.288.

Techniques: Expressing, Cell Counting, Comparison

Journal: Nutrients

Article Title: Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV- neu T) in Mice with Transplanted Salivary Gland Carcinoma Cells

doi: 10.3390/nu12051417

Figure Lengend Snippet: Cytotoxic T cells infiltrating tumors in BALB- neu T mice after treatments. Tumor tissues from three mice in each group were analyzed with IHC for CD8 expression. ( A ) Positive cell count/field averaging 10 representative microscopic fields (mean ± SD, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; one-way-ANOVA, Tukey’s multiple comparison). ( B ) Representative digital images (20×), scale bar represents 100 µm. T C : cytotoxic T lymphocytes, CUR: curcumin.

Article Snippet: Cytokine release, IHC data, and distribution of cell survival were preliminarily verified using the Kolmogorov–Smirnov test and data sets were analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s test with GraphPad Prism 5.00.288.

Techniques: Expressing, Cell Counting, Comparison

Journal: Nutrients

Article Title: Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV- neu T) in Mice with Transplanted Salivary Gland Carcinoma Cells

doi: 10.3390/nu12051417

Figure Lengend Snippet: Apoptotic cells within the tumors of BALB- neu T-treated mice. Tumor tissues from three mice in each group were analyzed by IHC analysis for cleaved caspase-3 expression (arrow). ( A ) Positive cell count/field averaging 10 representative microscopic fields (mean ± SD, *** p ≤ 0.001; one-way-ANOVA, Tukey’s multiple comparison). ( B ) Representative digital images (40×), scale bar represents 50 µm. CUR: curcumin.

Article Snippet: Cytokine release, IHC data, and distribution of cell survival were preliminarily verified using the Kolmogorov–Smirnov test and data sets were analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s test with GraphPad Prism 5.00.288.

Techniques: Expressing, Cell Counting, Comparison

Journal: Nutrients

Article Title: Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV- neu T) in Mice with Transplanted Salivary Gland Carcinoma Cells

doi: 10.3390/nu12051417

Figure Lengend Snippet: In vitro effect of mAb 4D5 on Head and neck cancer (HNC) cells. ( A ) FACS analysis was performed on HNC cell lines of the tongue (SCC-15), pharynx (FaDu), or salivary gland (A-253) using mAb 4D5 (5 μg/mL). MOPC-21 (5 μg/mL) was used as a control. ( B ) Cell growth was assessed by a sulforhodamine B (SRB) assay after 48 and 72 h of treatment with mAb 4D5 at different concentrations (1.25, 2.5, 5 μg/mL). MOPC-21 (5 μg/mL) was used as a control (CTRL). The results are expressed as the mean ± SD of three independent experiments performed in triplicate (* p ≤ 0.05, *** p ≤ 0.001, compared with the control cultures; one-way-ANOVA, Tukey’s multiple comparison).

Article Snippet: Cytokine release, IHC data, and distribution of cell survival were preliminarily verified using the Kolmogorov–Smirnov test and data sets were analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s test with GraphPad Prism 5.00.288.

Techniques: In Vitro, Control, Sulforhodamine B Assay, Comparison